SubtiBank SubtiBank
bkdAA [2020-09-03 17:02:58]
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bkdAA [2020-09-03 17:02:58]

2-oxoisovalerate dehydrogenase (E1 alpha subunit)
Locus
BSU_24050
Isoelectric point
4.78
Molecular weight
36.18 kDa
Protein length
330 aa Sequence Blast
Gene length
993 bp Sequence Blast
Function
utilization of branched-chain keto acids
Product
2-oxoisovalerate dehydrogenase (E1 alpha subunit)
Essential
no
E.C.
1.2.4.4
Synonyms
bfmBAA,bfmB1a,bkd
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of branched-chain amino acids

    • Gene

    Coordinates
    2,499,090 2,500,082

    The protein

    Catalyzed reaction/ biological activity

  • 3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase]-(R)-N6-lipoyl-L-lysine + H+ --> [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase]-(R)-N6-(S8-2-methylpropanoyldihydrolipoyl)-L-lysine + CO2 (according to UniProt)

    • Protein family

  • BCKDHA family (single member, according to UniProt)

    • Paralogous protein(s)

  • AcoA, PdhA

    • Structure

  • 1UMB (from Thermus thermophilus, 42% identity, 57% similarity) PubMed

    • Localization

  • Membrane-proximal (Spotty) PubMed

    • Expression and Regulation

    Operons

    Genes
    ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB
    Description
    PubMed

    Sigma factors

  • SigL: sigma factor, PubMed, in SigL regulon

    • Regulatory mechanism

  • BkdR: activation, PubMed, in BkdR regulon
  • CodY: repression, PubMed, in CodY regulon

    • Regulation

  • induced in the presence of isoleucine or valine (BkdR) PubMed
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    Biological materials

    Mutant

  • BKE24050 (bkdAA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCAAGCCCCTCCTTTAT, downstream forward: _UP4_TATGCGAAGTAGGGAGGAAG
  • BKK24050 (bkdAA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCAAGCCCCTCCTTTAT, downstream forward: _UP4_TATGCGAAGTAGGGAGGAAG

    • References

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